Protein Quantification (OD280)

Name: Protein Quantification (OD280)
Uploaded: 2010-03-02T03:42:38+00:00
Duration: 1 min 55 s
Channel: abnova
Description: by Abnova - Protein can be directly quantitated using UV absorbance (280nm). 280nm Absorbance depends on the Tyr and Trp (amino acids with aromatic rings) content. A very rough protein concentration can be obtained by making the assumption that the protein sample has an extinction coefficient of 1, so 1 OD = 1 mg/ml protein.

abnova

by Abnova - Protein can be directly quantitated using UV absorbance (280nm). 280nm Absorbance depends on the Tyr and Trp (amino acids with aromatic rings) content. A very rough protein concentration can be obtained by making the assumption that the protein sample has an extinction coefficient of 1, so 1 OD = 1 mg/ml protein.

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